U251（胶质瘤细胞）

产品名称：U251（胶质瘤细胞）
产品品牌：ATCC
价格：询价
上海研生生化试剂有限公司是一家专业经营ELISA 试剂盒、放免试剂盒、仪器耗材、科研细胞株的公司。 本产品U251，胶质瘤细胞相关详细信息请。
产品说明：
Subculturing:
 Protocol:

1.  Remove and discard culture medium.

2.  Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.  Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.   Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.   Add appropriate aliquots of the cell suspension to new culture vessels.

6.   Incubate cultures at 37°C.

Subc*tion ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium renewal: 2 to 3 times per week

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