PC-12分化(大鼠肾上腺嗜铬细胞瘤) 产品品牌:ATCC 价格:询价 我公司拥有国内的细胞服务技术、先进的仪器设备和专业的ELISA检测试剂盒, 生物素标记、荧光素标记、酶标记等,广泛用于多种分析研究与技术测定。 产品说明: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 相关产品: PC-12分化(大鼠肾上腺嗜铬细胞瘤) PA317(鼠胚胎成纤维细胞) SP2/0(骨髓瘤细胞) CHO(中国仓鼠卵巢细胞) COS-7(SV40转化的非洲绿猴肾细胞) VERO(非洲绿猴肾细胞)
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